Analysis of PSF of a Dipole Source by a High-NA Microscopy System
Abstract
The emission light of a fluorescent molecule and the scattered light of a nanosphere are well modeled by dipole sources. Therefore, a dipole source is a good model for a point source which considers the vectorial effects in practice. Analysis of the PSF of such point source is important. The dipole source is built in VirtualLab Fusion. By connecting with a complex high-numerical-aperture microscopy system, the PSF is directly calculated in VirtualLab Fusion.
VirtualLab Fusion Configuration
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VirtualLab Fusion
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Tutorial
Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
Use Case
Single-Molecule Imaging by High-NA Fourier Microscope
We model a complete high-NA Fourier microscopy system for single-molecule imaging. Exemplarily, we show the effect of e.g. Fresnel losses at interfaces, diffraction from apertures, and compare the simulation results to a reference.