Resolution Investigation for Microscope Objective Lenses by Rayleigh Criterion
Abstract
To characterize the resolution of a microscope, it is often practical to following the theory of “Rayleigh Criterion” which was invented by John William Strutt, 3rd Baron Rayleigh in 1896. The theory defines that when the center of one Airy pattern is just overlapped with the first minimum of another, then they can just be resolved. In this example, we follow Rayleigh’s theory and check the resolution for microscopy objective lenses with different numerical aperture (NA) values.
VirtualLab Fusion Configuration
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VirtualLab Fusion
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Tutorial
Usage of Debye-Wolf Integral Calculator
The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.
Tutorial
Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
Use Case
Investigation of Idealized Vectorial Focusing Situation Using Debye-Wolf Integral
We use the Debye-Wolf integral calculator in VirtualLab Fusion for vectorial focal field investigation with different parameters.