Single-Molecule Imaging with High-NA Fourier Microscope

Fourier microscopy is widely used for single-molecule imaging, surface plasma observation, photonic crystal imaging, etc. It enables the direct observation of the spatial frequency distribution. Different effects in the high-NA Fourier microscope (angle-dependent Fresnel losses at each lens surface, diffraction, etc.) can affect the final image quality obtained for the single molecule. The fast physical optics software VirtualLab Fusion can model the entire system with its powerful Field Tracing engine, including the Fresnel losses and aperture diffraction effects. An example is presented, and we compare the simulation results with experimental results from literature.