The management board has decided that all business trips until the end of June will be canceled without substitution. Unfortunately, this also means that we won’t attend any exhibitions until the end of June.

We demonstrate the resulting chromatic effects for a selected high-NA objective lens in VirtualLab Fusion. Additionally, we compare the results of the analysis for the real objective lens with the ideal situation obtained using the Debye-Wolf integral.

Fluorescent microscopy has proven to be a very effective technology for both biological and medical applications. When constructed in a reflective configuration, both the illuminating light and the light emitted by the fluorescent sample, which have different wavelengths, pass through the same objective lens of the fluorescent microscope. We take such an example and demonstrate the resulting chromatic effects for a selected high-NA objective lens in VirtualLab Fusion. Additionally, we compare the results of the analysis for the real objective lens with the ideal situation obtained using the Debye-Wolf integral.

With VirtualLab Fusion, we model a complete Fourier microscope system and use it for single-molecule imaging. Specifically, we demonstrate the influence of several physical-optics effects, including the Fresnel loss at each optical interface and the diffraction from lens apertures.

Fourier microscopy, in contrast to traditional imaging techniques, enables the direct observation of the spatial frequency distribution. Therefore, it is nowadays widely used for e.g. surface-plasma observation, photonic-crystal imaging, and so on. With VirtualLab Fusion, we model a complete Fourier microscope system and use it for single-molecule imaging. Specifically, we demonstrate the influence of several physical-optics effects, including the Fresnel loss at each optical interface and the diffraction from lens apertures.