Tight Focusing Through a Stratified Medium

In the real-life experiment, a coverslip is very often applied with a microscope for observing biological specimens. The focal spot of the high-NA objective could be influenced by the aberration introduced by the coverslip. In VirtualLab Fusion, the influence of the focal spot by the coverslip can be analyzed straightforwardly with a stratified medium. The aberrated focal spot is demonstrated and analyzed in this use case.