Microscopy System with Structured Illumination
Abstract
The microscopy system with structured illumination for fluorescent samples can improve the resolution of the microscopy system by a factor of 2 compared with the resolution predicted by Abbe theory. VirtualLab Fusion provides a fast way to investigate the structured illumination pattern by the incident wave property. The polarization of the incident wave and its influence on the contrast of the structured illumination pattern is investigated.
VirtualLab Fusion Configuration
-
VirtualLab Fusion
Are you interested in further reading?
Tutorial
Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
Tutorial
Usage of Debye-Wolf Integral Calculator
The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.
Use Case
Resolution Investigation for Microscope Objective Lenses by Rayleigh Criterion
Following the Rayleigh criterion, we investigate the resolution of three microscope objective lenses with different numerical apertures.