Diffraction from the Aperture in a Microscopy System
Abstract
In microscopy systems, the influence of the diffraction from the aperture in the system may have an effect on the PSF. However, it is rarely considered in the design as well as the usage. VirtualLab Fusion provides a straightforward way to include the diffraction effect in the microscopy lens system. This use case investigates the influences on the energy density at the entrance pupil of the tube lens as well as at the image plane.
VirtualLab Fusion Configuration
-
VirtualLab Fusion
Are you interested in further reading?
Tutorial
Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
Tutorial
Usage of Debye-Wolf Integral Calculator
The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.
Use Case
Resolution Investigation for Microscope Objective Lenses by Rayleigh Criterion
Following the Rayleigh criterion, we investigate the resolution of three microscope objective lenses with different numerical apertures.