Diffraction from the Aperture in a Microscopy System

In microscopy systems, the influence of the diffraction from the aperture in the system may have an effect on the PSF. However, it is rarely considered in the design as well as the usage. VirtualLab Fusion provides a straightforward way to include the diffraction effect in the microscopy lens system. This use case investigates the influences on the energy density at the entrance pupil of the tube lens as well as at the image plane.