Chromatic Effect Analysis in Fluorescent Microscopy
Abstract
Chromatic aberration plays an important role in the reflection-type fluorescent microcopy, because the emitting wavelength and illumination wavelength are different. On the other hand, such microscopy systems often employ high-NA lenses as objectives. Therefore,the vectorial effect must be also taken into consideration for the performance analysis. In VirtualLab Fusion, chromatic effects of high-NA objective lens can be analyzed in a full vectorial manner. As an example, the performance of a patented objective lens is evaluated.
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Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.