Analysis of Off-Axis Imaging by a High-NA Microscope

The off-axis PSF for imaging often suffers from the aberration of the microscopy system. It makes the microscopy system not perfectly shift-invariant as expected. VirtualLab Fusion provides a fast and convenient way to check the PSF for off-axis imaging with a high-NA microscope. This use case demonstrates the imaging of off-axis object points with different lateral shift distances, to check the influence of the aberrations.