Analysis of Off-Axis Imaging by a High-NA Microscope

The off-axis PSF for imaging systems often suffers from aberrations introduced by the plied optical components (e.g. a microscope system). Hence, the focus is not perfectly invariant to shifts as ideally expected. VirtualLab Fusion provides a fast and convenient way to check the light propagation and PSFs for off-axis imaging with a high-NA microscope. This use case demonstrates the imaging of off-axis object points with different lateral shift distances, to check the influence of the aberrations.