Analysis of Off-Axis Imaging by a High-NA Microscope

Abstract

The off-axis PSF for imaging systems often suffers from aberrations introduced by the plied optical components (e.g. a microscope system). Hence, the focus is not perfectly invariant to shifts as ideally expected. VirtualLab Fusion provides a fast and convenient way to check the light propagation and PSFs for off-axis imaging with a high-NA microscope. This use case demonstrates the imaging of off-axis object points with different lateral shift distances, to check the influence of the aberrations.

VirtualLab Fusion Configuration

  • VirtualLab Fusion VirtualLab Fusion

Are you interested in further reading?

Tutorial

Usage of Debye-Wolf Integral Calculator

The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.

Tutorial

Analyzing High-NA Objective Lens Focusing

High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.

Use Case

Resolution Investigation for Microscope Objective Lenses by Rayleigh Criterion

Following the Rayleigh criterion, we investigate the resolution of three microscope objective lenses with different numerical apertures.

Tutorial

Analysis of Focal Plane Position as a Function of Numerical Aperture

We analyze how varying different parameters of the system can affect the position of the focal plane.