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Reflecting Microscope System with very high Numerical Aperture


In single-molecule microscopy imaging applications, localization precision is a critical issue. Since the localization precision in a certain direction is proportional to the width of the point spread function (PSF) of the image in the same direction, a microscope with a higher numerical aperture (NA) can reduce the width of the PSF and thus improve the localization precision. In this use case, we demonstrate the modeling of very compact reflecting microscope system with an NA of 0.99 (Inagawa et al., 2015), and compare the results obtained with VirtualLab Fusion’s fast physical optics technology to the reference.

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