Tight Focusing by a High-NA Immersion Microscope
Abstract
In an immersion microscope, there is often a coverslip which separates the immersion liquid and the specimen. Therefore, the PSF could be distorted by the interface of the coverslip at the focal plane, which is not very often considered well in the design procedure. In VirtualLab Fusion, the influence of the PSF by the interface of the coverslip can be analyzed straightforwardly. The distortion of the focal spot behind the coverslip is demonstrated and analyzed in a fully vectorial manner.
VirtualLab Fusion Configuration
-
VirtualLab Fusion
Are you interested in further reading?
Tutorial
Analyzing High-NA Objective Lens Focusing
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
Tutorial
Usage of Debye-Wolf Integral Calculator
The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.
Use Case
Resolution Investigation for Microscope Objective Lenses by Rayleigh Criterion
Following the Rayleigh criterion, we investigate the resolution of three microscope objective lenses with different numerical apertures.