Fourier microscopy, in contrast to traditional imaging techniques, enables the direct observation of the spatial frequency distribution. Therefore, it is nowadays widely used for e.g. surface-plasma observation, photonic-crystal imaging, and so on. With VirtualLab Fusion, we model a complete Fourier microscope system and use it for single-molecule imaging. Specifically, we demonstrate the influence of several physical-optics effects, including the Fresnel loss at each optical interface and the diffraction from lens apertures.
Single Molecule Imaging by Fourier Microscopy
We model a complete high-NA Fourier microscope system for single-molecule imaging. Especially, we show the effect from e.g. Fresnel losses, diffraction due to apertures, and compare the simulation results against a reference.
High-NA objective lenses are widely used in optical lithography, microscopy, etc. Consideration of the vectorial nature of light in the simulation of the focusing is of great importance.
