During the conference we will give a talk on:
Numerical Analysis of Tightly Focused Spot for Confocal Microscopy Illumination by a Real Lens System
In this work, we perform the physical-optics modeling of the real condenser lens system with inclusion of full vectorial effects. The schematic of the illumination part of the confocal microscope is shown in the figure. The Gaussian wave source is generated in a fiber with a relative low divergence. It is collimated by a collimating lens (Edmund Optics: 49664) and then focused by a high numerical aperture condenser lens (Patent: US04384765; NA=0.95) to illuminate the sample.