Chromatic Effects in Fluorescent Microscopy

Fluorescent microscopy has proven to be a very effective technology for both biological and medical applications. When constructed in a reflective configuration, both the illuminating light and the light emitted by the fluorescent sample, which have different wavelengths, pass through the same objective lens of the fluorescent microscope. We take such an example and demonstrate the resulting chromatic effects for a selected high-NA objective lens in VirtualLab Fusion. Additionally, we compare the results of the analysis for the real objective lens with the ideal situation obtained using the Debye-Wolf integral.

Chromatic Effect Analysis in Fluorescent Microscopy

By taking a fluorescent microscope as example, we analyze the chromatic effects for a high-NA objective lens, at the emitting wavelength and illumination wavelength, respectively.

Debye-Wolf Integral Calculator

The Debye-Wolf integral calculator in VirtualLab Fusion computes the vectorial field near focus based on an idealized model, when the exact specifications of the objective lens are not known.

Download free trial version

Download free trial version